FIG. 3.
Impα3 knockdown significantly inhibited the infection of CD4+ C8166 T cells with wild-type HIV-1. (A) Inhibitory effect of Impα3-KD on HIV-1 replication and progression in CD4+ C8166 T cells. A total of 0.5 × 106 Impα3-KD or ScRNA-transduced C8166 cells were infected with HIV-1 pNL4.3-GFP at an MOI of 0.02. At various days postinfection (x axis), the supernatant was collected, and HIV p24gag levels were measured in order to monitor virus replication. (B) After 4 days of infection, the infected (GFP-positive) Impα3-KD and ScRNA-transduced C8166 cells were visualized by fluorescence microscopy (left), and the viral protein p24gag was detected by Western blotting using an anti-p24 antibody (right). (C) Levels of expression of the CD4 receptor on the surfaces of Impα3-KD, ScRNA-transduced, or normal (Mock) C8166 T cells were observed by anti-CD4 staining and flow cytometry analysis.