FIG. 3.
Construction of recombinant baculoviruses. (A and B) The approximate locations of the herpesviral genes encoding the viral glycoproteins gH, gB, gK, and gD in the prototypical arrangement of the HSV-1 viral genome are shown. (C) The portions of gH, gB, gK, and gD expressed by the baculoviruses are gH at aa 1 to 792, gB at aa 30 to 748, gK at aa 1 to 82, and gD at aa 1 to 340. (D) Schematic of the gene cassettes expressing truncated gH792, gB748, gKa, and gD340 inserted in recombinant baculovirus genomes. The signal sequence of gB (aa 1 to 29) was replaced with the melittin signal sequence. The truncated gH, gD, and gK glycoproteins contain their native signal sequences. UL, unique long; US, unique short; TR, terminal repeat; IR, inverted repeat. Tn7R and Tn7L are transposon sites in the baculovirus bacmid. Pp10 and PPH are baculovirus promoters for the p10 and polyhedrin genes, respectively.