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. 2010 Jun 23;84(17):8596–8606. doi: 10.1128/JVI.00298-10

FIG. 4.

FIG. 4.

Expression and characterization of gB748, gH792, gD340, and gKa by recombinant baculoviruses. Sf9 cellular extracts obtained after infection with recombinant baculoviruses expressing gKa, gKa plus gB, gKa plus gH, and gKa plus gD were separated by SDS-PAGE, and the expression of gKa was detected by using anti-FLAG antibody (A), while the expression of gB, gD, and gH was detected by using anti-V5 antibody (B) in Western immunoblots. Replicate samples were also treated with Endo H. PgK denotes the putative precursor to gKa. Lane 1, uninfected Sf9 cell lysates; lane 2, lysates from Sf9 cells infected with a baculovirus expressing gKa alone; lane 3, lysates containing gKa plus gD; lane 4, lysates containing gKa plus gH; lane 5, lysates containing gKa plus gB. The locations of the major gB, gD, gH, and gKa species are shown. Dots demarcate the relative positions of the molecular mass ladder (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa).