Skip to main content
. 2010 Jun 23;84(17):8596–8606. doi: 10.1128/JVI.00298-10

FIG. 5.

FIG. 5.

Coimmunoprecipitation experiments. Sf9 cellular extracts obtained after infection with each of the recombinant baculoviruses expressing gKa in combination with either gB, gD, or gH were used in coimmunoprecipitation experiments using the anti-FLAG antibody to precipitate gKa and anti-V5 antibody immobilized on magnetic beads to immunoprecipitate either gB, gD, or gH. The presence of gKa, gB, gD, and gH was detected in Western immunoblots by using anti-FLAG antibody immobilized on magnetic beads (A, B, and C) or anti-V5 antibody (D and E). B and E are lighter exposures of immunoblots shown in A and D, respectively. C shows replicate samples shown in A treated with Endo H. Lane 1, uninfected Sf9 cell lysates; lane 2, lysates from Sf9 cells infected with a baculovirus expressing gKa alone; lane 3, lysates containing gKa plus gD; lane 4, lysates containing gKa plus gH; lane 5, lysates containing gKa plus gB. Heavy and light chains of immunoglobulin are represented by IgH and IgL, respectively. Dots demarcate relative positions of the molecular mass ladder (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa).