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. 2010 Jun 23;84(17):8596–8606. doi: 10.1128/JVI.00298-10

FIG. 6.

FIG. 6.

Baculovirus expression of gB truncations with gKa. Additional recombinant baculoviruses coexpressing gB500, gB364, and gB154 with gKa were constructed. The anti-FLAG antibody (A) or the anti-V5 antibody (B) was used to detect the expression of gKa and gB truncations in Western immunoblots. Lane 1, lysates containing gK alone; lane 2, lysates containing gKa plus gB154; lane 3, lysates containing gKa plus gB364; lane 4, lysates containing gKa plus gB500; lane 5, lysates containing gKa plus gB748. Dots demarcate the relative positions of the molecular mass ladder (250, 150, 100, 75, 50, 37, 25, 20, and 15 kDa).