TABLE 1.
Synthetic oligonucleotides used to construct recombinant baculoviruses
| Primer | Sequencea |
|---|---|
| gK-Flag-EcoRI-F | 5′-TGATCAGAATTCGCCATGCTCGCCGTCCGTTCC-3′ |
| gK-Flag-BamHI-R | 5′-TGATCAGGATCCGCAGATATGGGCGTGGTTGCG-3′ |
| gK-Flag-9His-HindIII-R | 5′-ATATATAAGCTTCTAGTGATGGTGATGGTGATGGTGATGATGACCGGTACGCTTGTCATCGTCATCCTTGTAGTC-3′ |
| gB-F | 5′-ACCATGCGCCAGGGCGCCCCCGC-3′ |
| gB-aa 748-R | 5′-CGCGCGCCCCAGGTCGCCCATC-3′ |
| gB-aa 500-R | 5′-GGAGGAGGTGGTCTTGATGCG-3′ |
| gB-aa 364-R | 5′-GCAGACCGACGGGCGCTTTGGCAC-3′ |
| gB-aa 154-R | 5′-GATGTTCTCCTTGAAGACCACCGC-3′ |
| gB-Mel-NheI-F | 5′-TATATAGCTAGCatgaaattcttagtcaacgttgcccttgtttttatggtcgtatacatttct tacatctatgccGCGGCTCCGAGTTCCCCCGGCACG-3′ |
| gH-F | 5′-ACCATGGGGAATGGTTTATGGTTC-3′ |
| gH-aa 792-R | 5′-GGCTAGCAAATGAATGACGGTTC-3′ |
| gH-NsiI-F | 5′-TATATAATGCATATGGGGAATGGTTTATGGTTC-3′ |
| gD-F | 5′-ACCATGGGaGGtGCTGCCGCCAGG-3′ |
| gD-aa 340-R | 5′-CATGTTGTTCGGGGTGGCCGG-3′ |
| gD-NheI-F | 5′-TATATAGCTAGCATGGGaGGtGCTGCCGCCAGG-3′ |
| pcDNA-KpnI-R | 5′-TATATAGGTACCCACAGTCGAGGCTGATCAGCG-3′ |
| pcDNA-SphI-R | 5′-TATATAGCATGCCACAGTCGAGGCTGATCAGCG-3′ |
a
Nucleotides in boldface type indicate restriction sites. Nucleotides in lowercase and italics code for the melittin sequence. Single lowercase letters depict nucleotide changes in gD inserted to facilitate primer synthesis without altering the amino acid sequence.