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. 2010 Jun 23;84(17):8484–8494. doi: 10.1128/JVI.00738-10

FIG. 4.

FIG. 4.

Nonshuttling UL84 BAC is defective for DNA replication. (A) Evaluation of supernatant viral DNA accumulation from wt HCMV BAC and nonshuttling UL84 BAC-transfected cells. HFF cells were transfected with BAC DNA, supernatant virus was isolated, and viral DNA was extracted at 3, 4, 5, and 8 days posttransfection and assayed for viral DNA accumulation by real-time PCR. The data are presented as fold increase in viral DNA accumulation over the amount found at day 3 posttransfection. Error bars are standard deviations for three separate experiments. (B) Evaluation of intracellular viral DNA accumulation from wt HCMV BAC-transfected and NS84 BAC-transfected cells. The data are presented as fold increase in viral DNA accumulation over the amount of day 2 DNA.