FIG. 2.

(A) Outline of the experimental procedure used to purify nuclear complexes associated with AAV Rep proteins. See Materials and Methods for details of the TAP steps with streptavidin and calmodulin resins. (B) Sequential analysis of the purified extracts. Proteins present either in total nuclear extracts or in the Rep-containing complexes after elution from the second calmodulin resin were resolved by SDS-PAGE, followed by silver staining (upper panels) or Western blotting using an anti-Rep antibody (lower panels). C, control infected HeLa cells; R, HeLaAAVtCR cells expressing Rep upon HSV-1 infection; wt, wt HSV-1; ΔUL30, HSVΔUL30.