Figure 9. Marker expression in XEN cells is stable over several passages when cells are grown under standard serum-containing medium.

qRT-PCR data comparing marker expression in XEN cells grown under standard serum-containing conditions to XEN cells grown in serum-free medium. Despite some spikes in marker expression at some passages, over the entire course of this experiment there were no obvious trends (either upward or downward changes in expression) in any of the markers assessed. In addition, while some markers were differentially expressed between the two culture conditions, there was also no obvious change in marker expression in the serum-free medium when assessed over the entire course of the experiment. This suggests that XEN cells are stable when grown in culture over several passages.