FIGURE 1.

The acetonitrile catabolic pathway in R. jostii RHA1. A, the anh gene cluster located on pRHL2. The anhE gene is black. Genes encoding the ANHase subunits (anhA and anhB) are shaded in dark gray, and a probable cobalt transporter (anhT) is shaded light gray. The portion of the anhE gene that was deleted is indicated below the cluster. Upstream and downstream fragments of anhE are shown in dark gray and arrows with restriction enzyme sites represent primers used to construct a deletion mutant. B, the deduced acetonitrile catabolic pathway. C, the growth of wild type RHA1 (WT), the anhE deletion mutant (ΔanhE), and the complemented mutant on acetonitrile and acetamide (ΔanhEC).