FIGURE 2.

The assembly of ANHase in vitro. A, the portion of the anh gene cluster used to construct pETAnhA and pETAnhAE. B, the specific activity of ANHase reconstituted using cell extracts containing AnhA or AnhAE. Cells were grown in media supplemented with the indicated concentrations of CoCl₂. C, the specific activity of ANHase reconstituted using purified AnhA. AnhA was purified from cells grown in media supplemented with the indicated concentrations of CoCl₂. In the assembly experiments, purified AnhB was incubated with either cell extracts or purified AnhA. The concentration of CoCl₂ supplemented in the growth media is indicated in B and C. Error bar represents S.E.