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. 2010 Jun 17;285(33):25126–25133. doi: 10.1074/jbc.M110.109223

FIGURE 4.

FIGURE 4.

Electronic absorption spectrum of cobalt-bound AnhE. The spectra of 500 μm CoCl2 and the difference spectrum for AnhE titrated with 1 eq of CoCl2 are shown. The difference spectrum was generated by subtracting the spectrum of apo-AnhE (20 mm HEPES, pH 7.5, at 25 °C).