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Proposed mechanisms of the influence of Co2+ binding on the activity of AnhE. A, Co2+ binds to high and low affinity sites at the AnhE homodimer interface. B, Co2+ binds away from the dimer interface but drives dimerization through conformational changes. Circular and square surfaces represent high and low affinity sites, respectively. The implied equivalence of the two sites in the AnhE2(Co2+)2 species is unintended.
