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. 2010 Jun 15;285(33):25232–25242. doi: 10.1074/jbc.M110.144410

FIGURE 2.

FIGURE 2.

Reactions catalyzed by 2-KPCC. A, reduction of the redox active disulfide by NADPH. B, reduction of the redox active disulfide by DTT. C, mechanism of 2-KPC carboxylation. D, mechanism of inactivation of 2-KPCC by BES. The reduced form of the flavin thiol is shown as the thiolate to highlight the charge transfer, with increased absorbance at 540 nm, resulting from interaction with FAD. To experimentally observe maximal charge transfer in BES-modified 2-KPCC it was necessary to increase the pH from a value of 7.4 to 11.0.