FIGURE 4.

Noncompetitive rapid equilibrium inhibition of 2-KPCC-catalyzed 2-KPC oxidation by BES. Assays of 2-KPC carboxylation were performed as described under “Experimental Procedures.” The double-reciprocal plots for assays performed in the presence of different concentrations of BES are shown. Data points represent the average of duplicate experiments. The solid lines were generated by nonlinear least-square fits of the v versus S data to the equation for a rectangular hyperbola using Sigmaplot. Symbols: ●, 0 mm BES; ■, 2.5 mm BES; ▴, 5 mm BES; ▾, 7.5 mm BES.