FIGURE 2.

Production of GmCP8 in G. mellonella. The same amounts of proteins (60 μg) extracted from each tissue (A) and proteins (60 μg) of tissue culture supernatant (B) were subjected to Tricine-SDS-PAGE analysis (left panel), and each duplicate gel was utilized for immunoblot assays (right panel) using anti-GmCP8 Ab. CP8, 1 μg of GmCP8; Pl, plasma; Hc, hemocyte; Fb, fat body; Mg, midgut; It, integument. C, tissue-specific expression of GmCP8 mRNA. The GmCP8 mRNA was detected in the three tissues but not in the hemocytes. D, time course analysis of GmCP8 mRNA expression after E. coli injection. The GmCP8 mRNA was detected at constant levels in the fat body but not in the hemocytes. Corresponding actin bands for these samples are shown in the lower panel.