FIGURE 4.
Increased PtdCho synthesis after RA treatment in cells that overexpress CDP-choline pathway proteins. A, cell lines were incubated with 20 μm RA, and at the indicated times were pulsed with [2-14C]acetate for 4 h, and lipids were extracted, and the radiolabel associated with PtdCho was analyzed by thin layer chromatography and quantified by ImageQuant software as described under “Experimental Procedures.” B, graph shows the amount of PtdCho/cells obtained in Neuro-2a ± RA and in the indicated cell lines analyzed by thin layer chromatography and quantified by ImageQuant software. Graphs are representative of three independent assays ± S.E. Significance was determined comparing untreated Neuro-2a cells and each stable cell line with the Student's t test: *, p < 0.05.