FIGURE 2.

Rat sequences do not appear to have enhancer and silencer functions in the rat Insr gene. Schematic diagram of rat IR minigenes is shown on left. Gray boxes denote exons 10–12, and intervening lines indicate the introns in-between. Dotted lines indicate deletions. Numbers below the introns indicate sizes of deletions. Numbers above the lines indicate length of rat intron sequence. These minigenes were transfected into HepG2 and Fao cells, and IR minigene splicing was assessed as before. The mean ± S.E. for percent exon 11 inclusion in HepG2 (gray bar) and Fao cells (white bar) is shown as a bar graph on the right. Results are derived from at least three independent experiments and are given as mean ± S.E. Statistical significance was analyzed by ANOVA for each cell line independently. Bars with the same letter are not significantly different (p < 0.05).