FIGURE 3.

Caldecrin inhibits RANKL-stimulated calcineurin activity in (RANKL) RAW 264.7 cells. A, RAW264.7 cells were cultured and stimulated for 30 min by RANKL with (R+Cal) or without (RANKL) WT caldecrin (9 μg/ml). Cell homogenates were prepared, and calcineurin activities were compared (mean ± S.D. of three experiments; *, p < 0.05 versus RANKL-treated group). B, RAW264.7 cells were cultured as shown in A, and calcineurin contents in the cells prepared from unstimulated (−), RANKL-stimulated (R), or RANKL plus WT caldecrin (R+C) were compared by Western blotting with anti-calcineurin antibody.