FIGURE 2.

C1 domain of C-PMT targets plasma membrane. A, schematic representations of wild-type C-PMT (C-PMT WT) and the C-PMT variant deficient in the first four helices of the C1 domain (C-PMT ΔC1(4H)). B, intracellular localization of C-PMT wild type and C-PMT ΔC1(4H). C-PMT and C-PMT ΔC1(4H) were expressed in 293T cells and located by fluorescence microscopy (green). The plasma membrane region was visualized with anti-human CD-46 antibody (red), and the nucleus was visualized with 4′,6-diamidino-2-phenylindole (blue), respectively. Images are presented at the same magnification (scale bar, 10 μm). C, Western blot analysis for C-PMT wild type and C-PMT ΔC1(4H) in the cytoplasmic and membranous fractions of 293T cells. Equivalent amounts (10 μg protein/lane) of fractionated samples were subjected to 10% SDS-PAGE.