FIGURE 7.

C1 domain interacts with phospholipids. Binding of recombinant GST-C1(4H) (upper panel) to phospholipids was compared with binding of GST alone (lower panel) by surface plasmon resonance analyses. Purified recombinant GST-C1(4H) (100 nm) or GST (100 nm) was injected over the chip coated with the liposomes containing 30% PS, PG, PI, phosphatidylinositol 4,5-bisphosphate (PIP2), or PA. The composition of the liposomes was described under “Experimental Procedures.” PC, phosphatidylcholine.