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EM images of WT-actin and A167C, F169C, and S170C mutant actins. All actins but the F169C mutant form long helical filaments in the presence of 3 mm MgCl2. The F169C mutant actin alone and in the presence of cofilin forms short crooked filaments and filament aggregates, but long filaments of normal morphology are formed in the presence of phalloidin.
