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. 2010 Jun 10;285(33):25654–25665. doi: 10.1074/jbc.M109.092569

FIGURE 6.

FIGURE 6.

Exogenously applied 2,3-DHBA is a poor inducer of PR1 expression compared with SA. A, Arabidopsis seedlings harboring the SA-responsive PR1p::GUS reporter gene, grown for 2 weeks in liquid culture, were treated with increasing concentrations of SA or 2,3-DHBA for 24 h. GUS activity was determined in total extracts. The data represent the averages ± S.D. of four replicate samples normalized to total extractable protein. GUS activity was significantly different between SA and 2,3-DHBA at p < 0.05 (*). B, appearance of seedlings 24 h after treatment with SA or 2,3-DHBA. SA concentrations exceeding 100 μm are toxic (visible as tissue bleaching), whereas detrimental effects of 2,3-DHBA are apparent only at concentrations exceeding 300 μm.