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. 2010 Jun 1;285(33):25699–25707. doi: 10.1074/jbc.M110.124941

FIGURE 4.

FIGURE 4.

WRN coiled coil domain multimerizes with WRN in vivo and alters WRN function. A, immunoprecipitations (IP) of extracts from HeLa cells overexpressing FLAG-WRN(250–366); antibodies used for immunoprecipitation (above) and proteins detected are indicated (left), full length WRN (WRN-FL). B–D, colony formation assays comparing the survival of parental HeLa cells (solid black circles) with the survival of HeLa cells expressing FLAG-WRN(250–366) (open circles) to the indicated doses of camptothecin (B), 4-nitroquinoline 1-oxide (C), and hydroxyurea (D). All assays were carried out in triplicate; the average values are plotted, and the standard deviation (error bars) is indicated.