FIGURE 10.

Constitutive nuclear localization of Yap1 in tsa1Δ cells. A, GFP fluorescence was monitored in gpx3Δ cells and their isogenic wild type (BY4741) expressing GFP-fused Yap1 as described above, after treatment with 0.5 mm H₂O₂ or 20 μg/ml of edelfosine (see “Experimental Procedures”). Nuclear translocation of Yap1 was evident within 30 min of treatment of wild type cells with either H₂O₂ or edelfosine, whereas 60 min were required to detect its nuclear localization in gpx3Δ cells treated with edelfosine. No Yap1 nuclear localization was detected in gpx3Δ cells treated with 0.5 mm H₂O₂ up to 1 h. B, GFP fluorescence was monitored in untreated tsa1Δ cells and their isogenic wild type (BY4741) expressing GFP-fused Yap1, or C, untreated tsa1Δ cells expressing GFP-fused Yap1 carrying an empty pRS316 vector or containing the TSA1 gene.