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. 2010 Jun 10;285(33):25731–25742. doi: 10.1074/jbc.M110.122200

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — α-Tocopherol prevents changes in Pma1 localization induced by edelfosine and this depends on its antioxidant activity. A, localization of Pma1p-RFP in live cells (SEY6210-Pma1p-dsRFP) either untreated or incubated with edelfosine (20 μg/ml) alone or in combination with 50 μm α-TOC for 1 h. B, wild type cells (BY4741) either untreated or incubated with edelfosine (20 μg/ml) alone or in combination with 50 μm α-tocopherol or related compounds were fixed and processed for detection of Pma1p by immunofluorescence. Images acquired were analyzed and quantification was performed for each of the categories established in at least 100 cells per experimental condition. PM, plasma membrane; DIC, differential interference contrast.