FIGURE 8.

Nuclear translocation of Yap1 in response to edelfosine is prevented by α-tocopherol. GFP fluorescence was visualized in living wild type cells carrying pUG36-GFP-YAP1 either untreated or incubated for a short period of time (20 min) with edelfosine (see “Experimental Procedures”). A, wild type cells expressing the nucleolus marker Sik1 fused to RFP (endogenously tagged) were used. B, wild type (BY4741) cells carrying the pUG36-GFP-YAP1 were incubated for a short period of time (20 min) with edelfosine in the absence or presence of α-TOC (see “Experimental Procedures”). DIC, differential interference contrast.