FIGURE 9.

The oxidative stress response triggered by edelfosine differs from that induced by H₂O₂. A, deletion mutants for the indicated genes were compared with their isogenic wild type strain (BY4741). Cells were grown to mid-log phase and serial diluted onto rich medium + 0.2% ethanol (control) or rich medium containing edelfosine (10 μg/ml). B, strains lacking peroxiredoxins Gpx3 and Tsa1 were compared with their isogenic wild type strain (BY4741). Cells were grown to mid-log phase and serial diluted onto rich medium + 0.2% ethanol (control) or rich medium containing edelfosine (10 μg/ml) or H₂O₂ (4 mm). C, tsa1Δ cells carrying the pRS316 vector (empty vector) or containing TSA1 under its own promoter were compared with its isogenic wild type carrying the empty vector. Cells were grown to mid-log phase and serial diluted onto selective medium ± 5 μg/ml edelfosine. In all cases plates were incubated for 4 days at 25 °C.