TABLE 2.
Summary of equilibrium dissociation constants for CP binding to V-1 and barbed ends
Kd values between V-1/CP were calculated from fluorescence anisotropy data, and those for CP binding to barbed ends were calculated from pyrene actin fluorescence experiments. Data were fitted as described previously (60), and errors represent the S.D. from three independent experiments.
| CP |
Kd |
|
|---|---|---|
| V-1 | Actin | |
| nm | ||
| α1β2 | 14 ± 6 | 0.1 ± 0.08 |
| α1β2(K181E) | 16 ± 6 | 2.4 ± 0.4 |
| α1β2(L258K,L262K,V263K,A265K,L266K) | 14 ± 5 | 1.0 ± 0.2 |
| α1(R259E,R260E Q261E)β2 | 66 ± 12 | 6.3 ± 0.8 |
| α1β2(K142E,K143E) | 222 ± 40 | 1.9 ± 0.3 |
| α1(ΔC28)β2 | 68 ± 12 | 258 ± 168 |
| α1(ΔC10)β2 | 7 ± 2 | 0.91 ± 0.09 |