FIGURE 4.

The occupancy of Mondo-MLX on the Txnip promoter is reduced by NaN₃ or rotenone. A, the protein level of ectopic GFP-MondoA and FLAG-MLX or endogenous ChREBP was not affected by NaN₃ or rotenone treatment (1.5 h) in HeLa or INS-1 cells, whereas Txnip expression was repressed. B and C, ChIP assays in HeLa cells, respectively, treated (1 h) with NaN₃ and rotenone, using antibodies corresponding to the indicated proteins. NF-YA, nuclear factor Y, subunit A; Pol II, RNA polymerase II. D, ChIP assay in pancreatic INS-1 cells treated with NaN₃ (1 h) using antibodies corresponding to the indicated proteins. The occupancy was scored by quantitative real-time PCR, expressed as percentages of input total chromatin DNA. Error bars in B and D indicate S.E.