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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Host Microbe. 2010 May 20;7(5):354–361. doi: 10.1016/j.chom.2010.04.007

Fig. 1. PKR is required for IFN-α/β production by BM-DCs in response to EMCV, TMEV and SFV.

Fig. 1

BM-DCs (5×105/well) were infected with RNA viruses as indicated and IFN-α/β accumulation in the culture supernatants was measured by ELISA after overnight culture. Data are the mean ± SD of triplicate wells. (A) Production of IFN-β protein (left panel) and IFN-α protein (right panel) by BM-DCs infected with EMCV (MOI=10) or influenza virus (Flu (ΔNS1)) (MOI=1). Data are representative of at least three (influenza virus) and ten (EMCV) independent experiments. (B) Production of IFN-β protein (left panel) and IFN-α protein (right panel) by BM-DCs infected with TMEV(ΔL) (MOI=10) or SeV (MOI=1). Data are representative of at least three (TMEV) and ten (SeV) independent experiments. (C) IFN-α/β production in response to SFV is mainly dependent on MDA5. BM-DCs lacking either MDA5, RIG-I or PKR were infected with SFV (MOI=10). Data shown are one out of two experiments with similar results. (D) IFN-α production in relation to infectious dose for EMCV (left panel) and SeV (right panel). Data represent one of two experiments with similar results.