Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Host Microbe. 2010 May 20;7(5):354–361. doi: 10.1016/j.chom.2010.04.007

Fig. 3. Phosphorylation of eIF-2α is not necessary for IFN-α/β production in response to PKR-dependent viruses.

Fig. 3

(A) Confocal analysis of eIF-2α phosphorylation in Ser(51) mutant MEFs (eIF2A/A) and control MEFs. Cells were stimulated with poly I:C for 6 hours and stained with mAbs specific for phospho-eIF-2α and dsRNA (K1), as well as the nuclear dye DRAQ5. Phospho-eIF-2α and dsRNA staining are shown in green and red, respectively. (B) IFN-β protein (left panel) and IFN-α protein (right panel) from eIF-2A/A and control eIF-2S/S MEFs infected with EMCV (MOI=10), SFV (MOI=10) or SeV (MOI=1). Data shown are from one out of three experiments.