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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Host Microbe. 2010 May 20;7(5):354–361. doi: 10.1016/j.chom.2010.04.007

Fig. 4. PKR deficiency disproportionately reduces IFN-β protein versus mRNA.

Fig. 4

BM-DCs (5×105/well) were infected with RNA viruses for 12 hours as indicated. IFN-β protein in the culture supernatants and IFN-β mRNA in total cDNA from lysed cells (synthesized using random hexamer primers) were measured by ELISA and quantitative PCR, respectively. Data are the mean ± SD of triplicate wells. (A) Production of IFN-β protein and (B) IFN-β mRNA from DCs lacking MDA5, RIG-I or PKR following infection with EMCV (MOI=10), SFV (MOI=10) or SeV (MOI=1). Data are representative of two independent experiments with similar results.