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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Methods. 2010 Feb 17;51(3):277–288. doi: 10.1016/j.ymeth.2010.02.011

Fig. 8.

Fig. 8

Biotin–streptavidin block assay in the determination of UvsW translocation directionality. (A1 and B1) Schematic representation of the translocation of UvsW helicase on the biotin–streptavidin bearing DNA. Translocation of the enzyme away from the biotin–streptavidin block leads to rapid dissociation from the end of the DNA, whereas dissociation is prevented when translocation occurs in the direction of the block. ATPase activity of UvsW as a function of the 5′-biotin-labeled 30mer (A2) and 3′-biotin-labeled 30mer (B2) in the presence (closed circles) and absence (open circles) of streptavidin (1 μM).