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. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: Circ Res. 2010 Jun 10;107(3):365–373. doi: 10.1161/CIRCRESAHA.110.221259

Figure 5. Effect of 5-aza-2’-deoxycytidine on methylation of SP1 sites and SP1 binding.

Figure 5

H9c2 cells were treated with 1% O2 and 21% O2 for 24 hours in the absence or presence of 30 μM 5-aza-2’-deoxycytidine (5-Aza). (A) Methylation of SP1 binding sites was determined by methylation-specific PCR. (B) SP1 binding was determined by ChIP assays. Data are means ± SEM. * P < 0.05 vs. control (n = 4–9).