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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Protein Pept Lett. 2009;16(4):437–443. doi: 10.2174/092986609787848135

Figure 4.

Figure 4

Blot overlay assay to determine GD binding to anionic FPLC fractions or to highly purified eggshell matrix. (A) FPLC fractions 1 – 4 from OR and pipe-minus were incubated with GD or control medium as indicated prior to detection of bound GD using a polyclonal anti-GD antibody. Below the main blots are lighter exposures showing just the relative reactivity with the ~50 kDa anionic species. (B) Purifed eggshell matrix from OR ovaries was blotted and reacted with GD media overlay (lane 1) or control media overlay (lane 2) prior to detection of bound GD, or with 10E4 anti-heparan sulfate antibody (lane 3). Positions of the major ~50 kDa (**) and ~85 kDa (*) bands discussed in the text are indicated.