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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Neuroendocrinol. 2010 Jul;22(7):743–753. doi: 10.1111/j.1365-2826.2010.02034.x

Figure 2. Development of the nasal placode/GnRH-1 neurons.

Figure 2

Camera lucida drawing from E10.5-E12.5 mice embryos showing invagination of the nasal placode and the development of the respiratory epithelium (re), olfactory epithelium (oe) and presumptive VNO (pvno)/nasal pit (np). tv=telencephalon, III=third ventricle, IV=fourth ventricle, t=tongue, fb=forebrain. A. Parasagittal section immunocytochemically stained for Hu (early neuronal marker) shows many positive cells within the nasal placode at E10.5. B. Parasagittal section from an E11.5 GnRH-GFP mouse immunocytochemically stained for GFP shows positive cells within the presumptive VNO (pvno) C. Parasagittal section from an E12.5 mouse, immunocytochemically stained for GnRH-1 (C) shows GnRH-1 cells leaving the VNO (arrows) D. Parasagittal section from an E12.5 mouse, immunocytochemically stained for GnRH-1 (brown) and peripherin (blue) shows GnRH-1 cells associated with peripherin fibers crossing the nasal forebrain junction (NFJ), peripherin axons entering the developing olfactory bulb (OB) and GnRH-1 cells still associated with a subset of peripherin axons turning caudally towards the hypothalamus.