Fig. 2.
Effect of mGSTA4 transfection on nitrite production triggered by serum depletion and 4-HNE treatment. Aliquots of cells from WT, VT/mGSTA4-transfected MS1 cells were incubated at a density of 1×105 cells/ml DMEM in 24-well plate until near confluent. Nitrite production was measured in these cells in serum free medium without 4-HNE treatment and after 2 h treatment with 20 μM 4-HNE in serum free medium. The values are the mean±S.E. of three determinations and are representative of three separate experiments. *p<0.001 compared to the value as shown in WT or VT-transfected cells.