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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Toxicol Appl Pharmacol. 2008 Apr 7;230(2):187–196. doi: 10.1016/j.taap.2008.03.018

Fig. 6.

Fig. 6

ROS formation was attenuated by overexpression of mGSTA4-4 in endothelial cells. VT/mGSTA4-transfected MS1 cells (1×105) were cultured into Lab-Tek chambered coverglass (Nalge Nunc International, NY, USA) until near confluent. Confocal images were taken with a LSM 510 UV META inverted microscope (Carl Zeiss, Jena, Germany) with 63× Plan Apo chromatic objectives. The upper panel and lower panel are VT-transfected and mGSTA4-transfected endothelial cells, respectively. A and D are control cells without 4-HNE treatment; B and E show 4-HNE (20 μM) treatment for 2 h; C and F show 4-HNE treatment for 4 h. DCDHF-DA (ALEXIS Biochemicals, CA) was used to detect the ROS, including H2O2 and peroxynitrite, formation. The results are representative of two reproducible experiments.

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