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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Res. 2010 Jun 22;70(13):5628–5638. doi: 10.1158/0008-5472.CAN-10-0595

Figure 1.

Figure 1

RAGEv1 is secreted by tumor cells and binds RAGE-ligand. (A) Western blot analysis was performed to assess the secretion of RAGEv1 from C6 glioma cells stably transfected with empty vector control (mock) or RAGEv1. Total cell lysate or conditioned media were analyzed for RAGEv1 expression with antibodies to human RAGE or the specific RAGEv1 C-terminus epitope. GAPDH was used as a loading control for total cell lysate. (B) Binding of RAGEv1 to RAGE-ligands was assessed by incubation of GST-tag or GST-tagged s100A12 with conditioned media from mock and RAGEv1 transfected cells. Pulldown assays were performed to assess RAGEv1 binding and western blot analysis performed with antibodies against RAGEv1 on the elutant from pulldown reactions.