RAGEv1 inhibits tumorigenesis through blocking a JNK-dependent signaling mechanism. (A-C) Cells were incubated with control (DMSO) or inhibitors for SAPK/JNK (SP600125), MEK1/2 (U0126), p38 (SB203580) and AKT (Triciribine) for 1h before stimulation with RAGE-ligand (s100B). RNA was then extracted and QPCR performed for APAF1, PDGFB and TNF, normalized to GAPDH levels. (D) JNK inhibition blocks RAGE-ligand induced tumor cell invasion. Matrigel invasion assays were performed with mock transfected cells, treated with control (DMSO) or SAPK/JNK (SP600125) and in response to vehicle or RAGE-ligand (s100B). Data are means ± s.e.m from three independent experiments. Significant differences (P ≤ 0.05) between groups are indicated by an asterisk.