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Effects of doxorubicin and doxazolidine on decatenation of KDNA (kinetoplast DNA) by topoisomerase II. Lane 1, KDNA without topoisomerase II (catenated form); lane 2, KDNA with topoisomerase II (decatenated form); lane 3, doxorubicin, 500 nM; lane 4, doxorubicin, 50 nM; lane 5, doxazolidine, 500 nM; lane 6, doxazolidine, 50 nM. C: catenated DNA; N: nicked, decatenated DNA; R: relaxed, decatenated DNA.
