Figure 1.

Concept of the integrated oxidative folding. (a) μ-Conotoxin scaffold containing one diselenide bridge, one isotope-labeled disulfide bridge and one normal disulfide bridge. The formation of the diselenide bridge is thermodynamically preferred and improves the oxidative folding, while the formation of the labeled disulfide bridge is readily confirmed by detecting crossdisulfide NOEs. (b) Structures of μ-SIIIA and μ-selenoconotoxin analogs of SIIIA studied in this work.