Figure 4.
CSLM images of various live cells after incubation with peptide 4b (VS) at 1 μM for 2 h. A) COS-1 cells. B) NIH/3T3 fibroblasts. C) D1 astrocytes. D) PC-12 differentiated neurons. The top panel is an overlay of all fluorescence channels, while the bottom panel represents only the green channel for clarity. Hoechst 34850 was used to stain the nucleus blue and wheat germ agglutinin Alexa Fluor 594 conjugate was used to stain the plasma membrane red. The peptide is visualized as green fluorescence. Size bar represents 20 μm.