Deletion of p110α in SF1 neurons blunts the anorexigenic effects of central leptin. Five-month old chow-fed male mice (n=4 or 6/genotype) were matched for body weight, and received saline (1 μl, i.c.v.) at 16:00 followed by leptin (6 μg in 1 μl saline, i.c.v.) 24 hr later. Leptin-induced reductions in food intake (a), meal size (b), meal frequency (c) and RER (d) were monitored using the TSE metabolic chambers. (e) Leptin-induced weight loss was measured. Data are presented as mean ± SEM, and * P<0.05 and **P<0.01 between p110αlox/lox/SF1-Cre mice and p110αlox/lox mice.