Table 2.

Comparsion of B220^hi and B220^int B cells¹

	B220 hi	B220 int	P value
B6.56R/56R (n=12)
% of all B-cells	57 ± 4.3	42.9 ± 4.32
mean size	72.5 ± 1.0	64.5 ± 1.3	0.0001
% FO	39.0 ± 1.2	21 ± 2.2	0.0001
% MZ	56.2 ± 1.3	36.7 ± 3.3	0.0001
% CD21−,CD23−	4.1± 0.7	41.4 ± 4.7	0.0001
I-Ab (mfi)	883.1 ± 48.9	405.5 ± 39.6	0.0001
CD86 (mfi)	133.9 ± 12	122.3 ± 9.9	NS
B6.56R/+ (n=11)
% of all B-cells	65.2 ± 3.7	34 ± 3.82
mean size	71.7 ± 6.6	71.4 ± 0.4	NS
% FO	39.8 ± 2.2	21.7 ± 2.8	0.0001
% MZ	46.9 ± 2.0	37 ± 2.0	0.0012
% CD21−,CD23−	11.8 ± 0.9	38.5 ± 3.8	0.0001
I-Ab (mfi)	1050 ± 78.4	407.4 ± 39.6	0.0001
CD86 (mfi)	24.9 ± 4.9	37.5 ± 8	NS

¹Splenic cells from both strains were assayed by flow cytometry and B-cells were distinguished based on surface B220 levels. Tabulated are the surface phenotype and subset distribution data pertaining to these 2 B-cell types. P values pertain to comparisons of these 2 B-cell types using the Student’s t-test. NS means not significant.

²The percentage of B-cells that typed as B220^int I-A^b-int were significantly different in the 2 strains (P < 0.01).