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. 2010 Aug 10;4(8):e784. doi: 10.1371/journal.pntd.0000784

Figure 1. PCR to amplify E. granulosus antigen B genes from individual worms.

Figure 1

DNA fragments were amplified by PCR with different primer combinations (Table 1) using genomic DNA as templates which were isolated from a single MAW (panel A) and a single PSC (panel B). Lane B1, EgAgB1; lane B2, EgAgB2; Lane B3, EgAgB3; Lane B4-1, EgAgB4/1; Lane B4-2, EgAgB4/2; Lane B4-3, EgAgB4/3; Lane B5, EgAgB5; Lane B24, PCR products amplified with EgAgBF2 and EgAgB24URT, which was designed based on 3′ untranslated region sequences of EgAgB2 and EgAgB4. Panel C, Examples showing rapid PCR to determine the size of inserts before sequencing. Lane 1–4, EgAgB1–4 clones from PSC (ZGP5); lane 5–7, EgAgB3 clones from ZGP5 containing the second exon fragments; lane 8–11, EgAgB1–4 clones from MAW (ZGA2); lane 12–14, EgAgB3 clones from ZGA2 containing the second exon fragments. DNA makers (M) are shown to the left of the panels.