Table 1. Experimental design and RNA labeling.
Experiment group | Sample cell line (Cy5) | Control cell line (Cy3) |
Stable expression | BJAB-E1 | BJAB |
Inducible short-term expression | BJAB-tTA-E1 (−Tet) | BJAB-tTA-E1 (+Tet) |
Inducible long-term expression | BJAB-tTA-E1 (−Tet) | BJAB-tTA (−Tet) |
Tetracycline treatment | BJAB-tTA (−Tet) | BJAB-tTA (+Tet) |
RNA prepared from cells expressing EBNA-1 was labeled with Cy-5, while RNA from control cells with Cy-3. Labeled RNA was then co-hybridized on to individual microarray to capture the transcriptional changes induced in EBNA-1 expressing cells.