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. 2010 Aug 10;5(8):e12052. doi: 10.1371/journal.pone.0012052

Table 1. Experimental design and RNA labeling.

Experiment group Sample cell line (Cy5) Control cell line (Cy3)
Stable expression BJAB-E1 BJAB
Inducible short-term expression BJAB-tTA-E1 (−Tet) BJAB-tTA-E1 (+Tet)
Inducible long-term expression BJAB-tTA-E1 (−Tet) BJAB-tTA (−Tet)
Tetracycline treatment BJAB-tTA (−Tet) BJAB-tTA (+Tet)

RNA prepared from cells expressing EBNA-1 was labeled with Cy-5, while RNA from control cells with Cy-3. Labeled RNA was then co-hybridized on to individual microarray to capture the transcriptional changes induced in EBNA-1 expressing cells.