Figure 7. Structural arrangement of the γ complex subunits generated by molecular modelling.

(A) CCS trend-lines for various archetypal structure representations. The compact arrangement for the γ₄ (blue) is in the best agreement with experimental data (black line). (B) Steps for building a CG model structure of the full length γ subunit (i-iii) and higher oligomeric species of γ (iii-v). (i) CG model of the truncated γ based on the crystal structure. (ii) a model structure of the compact form of the truncated γ subunit. (iii) adding the missing residues to the truncated γ to produce the full length γ. Subunit domains I, II, III are represented by blue, red and silver spheres, respectively. The residues not present in the X-ray structure (domain IV) are depicted by a green sphere built by fitting inter-subunit angles (rotation and torsion angles) to the CCS data. Using this model of the γ monomer, CG model structures of γ₂, γ₃ and γ₄ are built systematically (iii-iv). (C) CG model structures for γ₂δ′, γ₃δ′ and γ₃δδ′ subcomplexes.